Detection of changes in avian influenza genome fragments by multitemperature single-strand conformational polymorphism

Gromadzka, B.; Smietanka, K.; Dragun, J.; Minta, Z.; Gora-Sochacka, A.; Szewczyk, B.

Molecular and Cellular Probes 22(5-6): 301-304


ISSN/ISBN: 0890-8508
PMID: 18957320
DOI: 10.1016/j.mcp.2008.09.003
Accession: 022368926

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We have applied the temperature-dependent single-stranded conformational polymorphism (SSCP) analysis for characterization of influenza A virus cDNA fragments. A series of primers were synthesized on the basis of the comparison of hemagglutinin and PB2 gene sequences of different origin. RT-PCR reactions were run using these primers, products were denatured and single-strand DNA fragments were subjected to electrophoresis. After silver staining, ssDNA band patterns characteristic for each genotype were observed. Minor differences within a serotype were detected without need for sequencing the PCR products making the preliminary analysis of avian influenza isolates much faster.