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Expression, activity, and pro-hypertrophic effects of PDE5A in cardiac myocytes


, : Expression, activity, and pro-hypertrophic effects of PDE5A in cardiac myocytes. Cellular Signalling 20(12): 2231-2236

Cyclic GMP-selective phosphodiesterase type 5 (PDE5) has been traditionally thought to play a little role in cardiac myocytes, yet recent studies using selective inhibitors such as sildenafil suggest it can potently modulate acute and chronic cardiac stress responses. To date, evidence for myocyte PDE5 expression and regulation has relied on small-molecule inhibitors and anti-sera, leaving open concerns regarding non-specific immune-reactivity, and off-target drug effects. To directly address both issues, we engineered a robust PDE5-gene silencing shRNA (inserted into miRNA-155 cassette) and DsRed–PDE5 fusion protein, both coupled to a CMV promoter and incorporated into adenoviral vectors. PDE5 mRNA and protein knock-down eliminated anti-sera positivity on immunoblots and fluorescent immuno-histochemistry in neonatal and adult cardiomyocytes, and suppressed PDE5 enzyme activity. Stimulation of myocyte hypertrophy by phenylephrine was blunted by PDE5 gene silencing in a protein kinase G dependent manner, and this effect was similar to that from sildenafil with no additive response by both combined. DsRed–PDE5 fusion protein expression showed normal z-band localization in adult myocytes but was diffused in eNOS−/− myocytes; echoing reported findings with anti-sera. PDE5 overexpression increased enzyme activity and amplified natriuretic peptide gene expression from phenylephrine stimulation. These data confirm PDE5 expression, activity, and targeted inhibition by sildenafil in cardiomyocytes, as well as the role of this PDE in cardiomyocyte hypertrophy modulation.

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Accession: 022622390

PMID: 18790048

DOI: 10.1016/j.cellsig.2008.08.012

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Other references

Johnston, M. C., 1964: Facial malformation in chick embryos resulting from removal of neural crest. J DENT RES 43(5 Pt 2): 822

Kocon, J., 1990: Structure and ultrastructure of the reaction wood of the Polish larch (Larix polonica Rac.) investigated with the electron microscope and X ray diffraction. SEM and Roentgen X-ray diffraction studies are reported of branchwood samples from a tree of Larix polonica [L. decidua] growing in the mountainous area of Ujsoy, Poland. Tracheid walls in spring wood were much thicker than in normal wood resultin...

Paquet, Vincent; Strul, John; Servais, Laurent; Pelc, Isidore; Fossion, Pierre, 2002: Sleep-related eating disorder induced by olanzapine. Journal of Clinical Psychiatry 63(7): 597, July

American Laryngological Association, 1964: Scientific papers of the American Laryngological Association, 1964. Selections from the 14 papers will be abstracted in relevant sections of BIOLOGICAL

Rothe, M., 1974: Present problems of modern flavour research. Nahrung 8 (2) 115-123

Jolivet P.; Kien P., 1992: Sulphite metabolization by wheat chloroplasts. The study of Na2SO3 metabolization by wheat (Triticum aestivum L.) intact chloroplasts evidenced oxidation to SO4-2 in fresh but not in boiled chloroplasts. Since this process was observed both in the light and dark, it cannot be accounted for mer...

Saleh, Mansoor N.; Sugarman, Steve; Murray, James; Ostroff, Joy B.; Healey, Diane; Jones, Dennie; Daniel, Carol R.; Lebherz, Donna; Brewer, Hannah; Onetto, Nicole; Lobuglio, Albert F., 2000: Phase I trial of the anti-Lewis Y drug immunoconjugate BR96-Doxorubicin in patients with Lewis Y-expressing epithelial tumors. Purpose: We conducted a phase I clinical trial of BR96-Doxorubicin (BR96-Dox), a chimeric anti-Lewis Y (LeY) monoclonal antibody conjugated to doxorubicin, in patients whose tumors expressed the LeY antigen. The study aimed to determine the toxici...

Rusznak, C.; Devalia, J. L.; Davies, R. J., 1995: Exposure to combination of 200ppb sulphur dioxide and 400ppb nitrogen dioxide increases the airway response of mild asthmatics to allergen inhalation in a time-lagged manner. European Respiratory Journal 8(SUPPL 19): 572S

Tisserant, C., 1955: Les Cochlospermum de 1 Quest Africain. Descriptions and geographical notes on Cochlospermum plan-choni, C. tinctorium, and C. intermedium in French West Africa.

Soljanto P.; Rehtijarvi P.; Tuovinen O. H., 1980: Ferrous iron oxidation by Thiobacillus ferrooxidans; inhibition by finely ground particles. Geomicrobiology Journal 2(1): 1-12