Fungitoxicity of Cymbopogon citratus and Cymbopogon martinii on Colletotrichum gloeosporioides in bell pepper fruits

Carvalho, J.B.; Schwan-Estrada, K.R.F.; Bonaldo, S.M.; Cruz, M.E.S.; Carlos, M.M.; Stangarlin, J.R.

Revista Brasileira de Plantas Medicinais 10(1): 88-93

2008


ISSN/ISBN: 1516-0572
Accession: 022688226

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Abstract
Anthracnose of bell pepper has provoked raised losses for the culture in the post harvest, when the disease is more severe. The use of alternative methods of post harvest disease control has been intensified to improve the reduction of the use of pesticides, and the use of medicinal plants can be an alternative in the disease control. This work aimed at the verification of the in vitro fungitoxic potential of the Cymbopogon citratus and Cymbopogon martinii as an alternative to control Colletotrichum sp. in bell pepper post harvest period. In vitro test, concentrations of 1, 5, 10, 15, 20, 25 and 50% of the crude extract (CE) autoclaved of the two species were incorporated into PDA. It was evaluated sporulation for the counting of spores in hemacytometer, and the spore germination and apressorio formation in ELISA as well. In the in vivo test bell pepper fruits were inoculated with spore suspension (1 x 10(5) spores mL(-1)) and after 2 days treated with 10 and 20% extract prepared for microwave irradiation. Distilled water and citric extract were used as controls. Fruits weight reduction and disease severity were evaluated. The CE from the two species have not significantly inhibited the mycelial growth, the sporulation and the spores germination, however it had inhibitory effect on the apressorio formation. During the in vivo assay, the disease was not controlled for the CE. In the parameter fruit weight reduction, the CE was similar to the positive control, however smaller fruits weight reduction was verified with C. martinii CE at a concentration of 10% concentration.