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High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar

Croxford, A.E.; Rogers, T.; Caligari, P.D.S.; Wilkinson, M.J.

New Phytologist 180(3): 594-607

2008


ISSN/ISBN: 0028-646X
PMID: 18684160
DOI: 10.2307/25150610
Accession: 022763886

* The provision of sequence-tagged site (STS) anchor points allows meaningful comparisons between mapping studies but can be a time-consuming process for nonmodel species or orphan crops. * Here, the first use of high-resolution melt analysis (HRM) to generate STS markers for use in linkage mapping is described.

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