+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Leaf-associated bacterial and fungal taxa shifts in response to larvae of the tree hole mosquito, Ochlerotatus triseriatus



Leaf-associated bacterial and fungal taxa shifts in response to larvae of the tree hole mosquito, Ochlerotatus triseriatus



Microbial Ecology 55(4): 673-684



Larvae of the eastern tree hole mosquito, Ochlerotatus triseriatus (Say), and related container-breeding species are known to feed upon substrate-associated microorganisms. Although the importance of these microbial resources to larval growth has been established, almost nothing is known about the taxonomic composition and dynamics of these critical microbial food sources. We examined bacterial and fungal community compositional changes on oak leaves tethered in natural tree hole habitats of O. triseriatus. We eliminated larvae experimentally in a subset of the tree holes and examined 16S rDNA gene sequences for bacteria and ergosterol concentrations and 18S rRNA gene sequences for fungi collected from leaf material subsamples. Leaf ergosterol content varied significantly with time, but not treatment. Principal component analysis (PCA) was used to compare microbial taxonomic patterns found in leaves incubated with or without larvae present, and we found that larval presence affected both bacterial and fungal groups, either from loosely attached or strongly adherent categories. Bacterial communities generally grouped more tightly when larvae were present, and class level taxa proportions changed when larvae were present, suggesting selection by larval feeding or activities for particular taxa such as members of the Bacteroidetes, Alphaproteobacteria, and Betaproteobacteria classes. Fungal taxa composite scores also separated along PC axes related to the presence of larvae and indicated larval feeding effects on several higher taxonomic groups, including Saccharomycetes, Dothideomycetes, and Chytridiomycota. These results support the hypothesis that larval mosquito feeding and activities altered microbial communities associated with substrate surfaces, potentially leading to decreased food value of the resource and affecting decomposition of particulate matter in the system.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 022937590

Download citation: RISBibTeXText

PMID: 17899246

DOI: 10.1007/s00248-007-9310-6


Related references

Leaf-Associated Bacterial and Fungal Taxa Shifts in Response to Larvae of the Tree Hole Mosquito, Ochlerotatus triseriatus. Microbial Ecology 55(4): 673-684, 2008

The Salivary Gland Transcriptome of the Eastern Tree Hole Mosquito, Ochlerotatus triseriatus. Journal of Medical Entomology 47(3): 376-386, 2010

The salivary gland transcriptome of the eastern tree hole mosquito, Ochlerotatus triseriatus. Journal of Medical Entomology 47(3): 376-386, 2010

Linking movement and oviposition behaviour to spatial population distribution in the tree hole mosquito Ochlerotatus triseriatus. Journal of Animal Ecology 77(1): 156-166, 2008

Bacterial community structure in tree hole habitats of Ochlerotatus triseriatus: influences of larval feeding. Journal of the American Mosquito Control Association 24(2): 219-227, 2008

Leaf litter and larval mosquito aedes triseriatus dynamics in tree hole ecosystems. Ecology (Washington D C) 63(2): 283-288, 1982

Effects of variation in quality of leaf detritus on growth of the eastern tree-hole mosquito, Aedes triseriatus. Canadian Journal of Zoology 75(5): 706-718, 1997

Gene map of the eastern North American tree hole mosquito, Aedes triseriatus equals Protomacleaya triseriatus. O'Brien, S J Genetic Maps: Locus Maps of Complex Genomes, Sixth edition, No 3 ; Lower eukaryotes : 260-263, 1993

Natural infection of the tree hole breeding mosquito Aedes triseriatus (Diptera: Culicidae) with the fungus Funicularius triseriatus. Journal of Invertebrate Pathology 342: 199-202, 1979

The bionomics of some tree-hole mosquitoes. I, Physico-chemical factors in relation to mosquito prevalence in rot-hole water, by BEATTIE; II, The food of mosquito larvae in tree-holes in Buckinghamshire, England, by HOWLAND. Bull Ent Res [london] 20(1): 45-58, 1929

Leaf scraping beetle feces are a food resource for tree hole mosquito larvae. American Midland Naturalist y; 150(1): 181-184, 2003

Leaf Scraping Beetle Feces Are a Food Resource for Tree Hole Mosquito Larvae. American Midland Naturalist 150(1): 181-184, 2003

Oviposition site selection by the tree-hole mosquito, Aedes triseriatus (Say). Journal of Medical Entomology 5(2): 189-194, 1968

Geography of larval dormancy in the tree-hole mosquito, Aedes triseriatus (Say). Canadian Journal of Zoology 596: 1014-1021, 1981

Oviposition site selection by the tree-hole mosquito, Aedes triseriatus (Say). Dissertation Abstracts, 28B 391 - 392, 1967