Section 24
Chapter 23,045

Mitochondrial DNA and microsatellite DNA as genetic tags for stocked population of black rockfish Sebastes inermis of hatchery origin

Murakami, T.; Aida, S.; Yoshioka, K.; Yoshida, H.; Blanco Gonzalez, E.; Nishibori, M.; Umino, T.

Nippon Suisan Gakkaishi y; 72(4): 710-716


DOI: 10.2331/suisan.72.710
Accession: 023044604

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Black rockfish juveniles (Sebastes inernis) obtained from five broodstock females in the hatchery were marked with alizarin complexon (ALC), and a total of 81,000 juveniles marked were released in the coastal waters of Ikuno Island, central Seto Inland Sea, western Japan. Variation of the mitochondrial DNA (mtDNA) control region sequence and a microsatellite DNA marker were investigated on 81 individuals caught at the same site a year later. Sixty-one mtDNA haplotypes were observed in 81 individuals and four individuals shared identical mtDNA sequence with broodstock females. The genotypic analysis on a single microsatellite DNA marker also showed that the four individuals shared the same allele with the female broodstocks, and that all individuals were found to carry ALC marking. These results indicate that genetic tags are useful as well as ALC marking as stock separation tools of black rockfish.

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