Molecular cloning of dihydroflavonol 4-reductase gene from grape berry and preparation of an anti-DFR polyclonal antibody
Zhang Ping; Wen PengFei; Wan SiBao; Wang Wei; Pan QiuHong; Zhan JiChen; Huang WeiDong
Vitis 47(3): 141-145
ISSN/ISBN: 0042-7500 Accession: 023055379
Dihydroflavonol 4-reductase (DFR, EC 126.96.36.199) is a key enzyme of the flavonoid pathway, which synthesizes numerous secondary metabolites to determine the quality of grape berry and wine. The full-length dfr cDNA with 1014 bp was cloned from grape berry, and then introduced into an expressed plasmid pET-30a (+) vector at the EcoR I and Xho I restriction sites.