Section 24
Chapter 23,056

Molecular cloning of dihydroflavonol 4-reductase gene from grape berry and preparation of an anti-DFR polyclonal antibody

Zhang Ping; Wen PengFei; Wan SiBao; Wang Wei; Pan QiuHong; Zhan JiChen; Huang WeiDong

Vitis 47(3): 141-145


ISSN/ISBN: 0042-7500
Accession: 023055379

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Dihydroflavonol 4-reductase (DFR, EC is a key enzyme of the flavonoid pathway, which synthesizes numerous secondary metabolites to determine the quality of grape berry and wine. The full-length dfr cDNA with 1014 bp was cloned from grape berry, and then introduced into an expressed plasmid pET-30a (+) vector at the EcoR I and Xho I restriction sites.

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