+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Multiphoton microscopic imaging of adipose tissue based on second-harmonic generation and two-photon excited fluorescence



Multiphoton microscopic imaging of adipose tissue based on second-harmonic generation and two-photon excited fluorescence



Scanning 30(6): 452-456



The fresh adipose tissue was investigated by the use of multiphoton microscopy (MPM) based on two-photon excited fluorescence and second-harmonic generation (SHG). Microstructure of collagen and adipose cells in the adipose tissue is clearly imaged at a subcellular level with the excitation light wavelengths of 850 and 730 nm, respectively. The emission spectrum of collagen SHG signal and NADH and FAD fluorescence signal can also be obtained, which can be used to quantify the content of collagen and adipose cells and reflect the degree of pathological changes when comparing normal tissue with abnormal adipose tissue in the same condition. The results indicate that MPM has the potential to be applied to investigate the adipose tissue and can be used in the research field of lipid and connective tissues.

(PDF emailed within 0-6 h: $19.90)

Accession: 023077657

Download citation: RISBibTeXText

PMID: 18942106

DOI: 10.1002/sca.20130


Related references

Multiphoton microscopic imaging of in vivo hair mouse skin based on two-photon excited fluorescence and second harmonic generation. Scanning 34(3): 170-173, 2012

Two-photon fluorescence and second-harmonic generation imaging of collagen in human tissue based on multiphoton microscopy. Scanning 33(1): 53-56, 2011

Jadassohn-Pellizzari anetoderma: study of multiphoton microscopy based on two-photon excited fluorescence and second harmonic generation. European Journal of Dermatology 19(6): 570-575, 2010

Nonlinear spectral imaging of human hypertrophic scar based on two-photon excited fluorescence and second-harmonic generation. British Journal of Dermatology 161(1): 48-55, 2009

Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation. Proceedings of the National Academy of Sciences of the United States of America 100(12): 7075-7080, 2003

Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence. Optics Letters 27(23): 2082-2084, 2007

In vitro imaging of thyroid tissues using two-photon excited fluorescence and second harmonic generation. Photomedicine and Laser Surgery 28 Suppl 1: S129-S133, 2010

Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence. Proceedings of the National Academy of Sciences of the United States of America 99(17): 11014-9, 2002

Simultaneous multichannel nonlinear imaging: combined two-photon excited fluorescence and second-harmonic generation microscopy. Micron 32(7): 685-689, 2001

Detection and imaging of non-contractile inclusions and sarcomeric anomalies in skeletal muscle by second harmonic generation combined with two-photon excited fluorescence. Journal of Structural Biology 162(3): 500-508, 2008

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals. Journal of Biomedical Optics 14(2): 020503, 2009

Minimally invasive imaging method based on second harmonic generation and multiphoton excitation fluorescence in translational respiratory research. Respirology 16(1): 22-33, 2011

Multiphoton excited hemoglobin fluorescence and third harmonic generation for non-invasive microscopy of stored blood. Biomedical Optics Express 7(9): 3449-3460, 2016

Optical visualization of Alzheimer's pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation. Optics Express 17(5): 3679-3689, 2009

Multiphoton fluorescence and second-harmonic-generation microscopy for imaging structural alterations in corneal scar tissue in penetrating full-thickness wound. Archives of Ophthalmology 125(7): 977-978, 2007