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Note on staining nematodes in root tissue

Note on staining nematodes in root tissue

Proceedings Helminthol Society Washington 8(1): 26

Goodey's method of staining is modified by dehydrating and clearing in the same soln., rather than running the material through alcohols and clove oil. The parasitized roots are boiled 1 min. in Goodey's stain: Phenol, pure crystals, 20 g.; Lactic acid (sp. gr. 1.31) 20 g.; Glycerine (sp. gr. 1.25) 40 g.; Distilled water 20 cc; Acid fuchsin or cotton blue (1 g. to 100 cc. water) 5 cc. After washing in tap water the tissue is dehydrated and cleared in the same soln., without the stain, for 1 to several days. The material may be kept for examination in the same soln. or mounted in glycerine.

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