Protease activation in the starfish Asterias forbesi
Tillinghast, E.K.; Levasseur, S.
Comparative Biochemistry and Physiology. B Comparative Biochemistry 50(1): 131-135
ISSN/ISBN: 0305-0491 PMID: 1122706 DOI: 10.1016/0305-0491(75)90312-0
1.1. The activation and release of proteases from homogenates and subcellular fractions of the pyloric cacae of the starfish Asterias forbesi was analyzed.2.2. Activation occurs rapidly at 37°C and only slightly at 5°C.3.3. Once released the proteases are not further activated.4.4. Proteases are readily solubilized at 5°C by urea, mercaptoethanol and deoxycholate.5.5. The combination of 1 mM HgCl2 and 2 mM l-ascorbate caused a rapid release of proteases at 5°C.