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Purification, characterization and crystallization of an extracellular alkaline protease from Aspergillus nidulans HA-10

Charles, P.; Devanathan, V.; Anbu, P.; Ponnuswamy, M.N.; Kalaichelvan, P.T.; Hur, B-Ki.

Journal of Basic Microbiology 48(5): 347-352

2008

ISSN/ISBN: 0233-111X
PMID: 18702069
DOI: 10.1002/jobm.200800043
Accession: 023440604
Aspergillus nidulans is a highly potent fungus used in the production of alkaline protease. Extracellular alkaline protease was purified from A. nidulans in a two-step procedure involving ammonium sulphate precipitation and Sephadex G-100 column chromatography. The molecular mass of the enzyme was determined to be 42 kDa by SDS-PAGE. The enzyme activity was also analyzed by zymogram with gelatin. The enzyme was more stable over a wide range of pH (6-10) and the temperatures up to 50 degrees C.

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