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Ras protein participated in histone acetylation-mediated cell cycle control in Physarum polycephalum

Li, X.; Lu, J.; Zhao, Y.; Wang, X.; Huang, B.

Chinese Science Bulletin ust; 50(16): 1721-1725

2005


DOI: 10.1360/982005-307
Accession: 023461117

In this paper, we demonstrate that in Physarum polycephalum, a naturally synchronized slime mold, histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), arrestes the cell cycle at the checkpoints of S/G2, G2/M and mitosis exit, and influences the transcription of two ras genes Ppras1 and Pprap1, as well as the Ras protein level. Antibody neutralization experiment using anti-Ras antibody treatment showed that Ras protein played an important role in cell cycle checkpoint control through regulation of the level of Cyclin B1, suggesting that Ras protein might be a key factor for histone acetylation-mediated cell cycle regulation in P polycephalum.

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