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Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line

Li, W.-D.; Zhang, B.-d.; Wei, R.; Liu, J.-H.; Lin, Z.-B.

Acta Pharmacologica Sinica 29(5): 620-627

2008


ISSN/ISBN: 1745-7254
PMID: 18430371
DOI: 10.1111/j.1745-7254.2008.00776.x
Accession: 023525467

To investigate the reversal effect of Ganoderma lucidum polysaccharides (Gl-PS) on multidrug resistance (MDR) in the adriamycin (ADM)-resistant leukemic cell line K562/ADM. Cytotoxicity was assayed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide method; the ADM concentration in cells was determined by flow cytometry and confocal laser scanning microscopy techniques; the expression of P-glycoprotein was assayed by flow cytometry; and the mRNA expression levels of MDR-1 and MDR-associated protein (MRP)1 were determined by RT-PCR. Gl-PS reversed MDR in K562/ADM cells. Gl-PS obviously reversed the resistance of K562/ADM to doxorubicin. The reversing factors of Gl-PS at 10 and 20 mg/L were 6.46 and 6.80, respectively. MDR-1 and MRP1 transcription were downregulated by 10 and 50 mg/L Gl-PS. Gl-PS can reverse the MDR by downregulating the expression of MDR-1 and MRP1 in K562/ADM cells.

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