+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The N protein of Tomato spotted wilt virus (TSWV) is associated with the induction of programmed cell death (PCD) in Capsicum chinense plants, a hypersensitive host to TSWV infection



The N protein of Tomato spotted wilt virus (TSWV) is associated with the induction of programmed cell death (PCD) in Capsicum chinense plants, a hypersensitive host to TSWV infection



Virus Research 137(2): 245-252



In sweet pepper, the Tsw gene, originally described in Capsicum chinense, has been widely used as an efficient gene for inducing a hypersensitivity response (HR) derived Tomato spotted wilt virus (TSWV) resistance. Since previously reported studies suggested that the TSWV-S RNA mutation(s) are associated with the breakdown of Tsw mediated TSWV resistance in peppers, the TSWV genes N (structural nucleocapsid protein) and NS(S) (non-structural silencing suppressor protein) were cloned into a Potato virus X (PVX)-based expression vector, and inoculated into the TSWV-resistant C. chinense genotype, PI 159236, to identify the Tsw-HR viral elicitor. Typical HR-like chlorotic and necrotic lesions followed by leaf abscission were observed only in C. chinense plants inoculated with the PVX-N construct. Cytopathological analyses of these plants identified fragmented genomic DNA, indicative of programmed cell death (PCD), in mesophyll cell nuclei surrounding PVX-N-induced necrotic lesions. The other constructs induced only PVX-like symptoms without HR-like lesions and there were no microscopic signs of PCD. The mechanism of TSWV N-gene HR induction is apparently species specific as the N gene of a related tospovirus, Tomato chlorotic spot virus, was not a HR elicitor and did not cause PCD in infected cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 023798733

Download citation: RISBibTeXText

PMID: 18722487

DOI: 10.1016/j.virusres.2008.07.020


Related references

The N protein of Tomato spotted wilt virus is associated with the induction of programmed cell death in Capsicum chinense plants, a hypersensitive host to TSWV infection. Virus Research7: 2, 245-252, 2008

Transgenic plants expressing a single-chain Fv antibody to Tomato spotted wilt virus (TSWV) are resistant to TSWV systemic infection. Acta Horticulturae (722): 337-348, 2006

Capsicum chinense PI 159236: a source of resistance to Phytophthora capsici and tomato spotted wilt virus (TSWV). Capsicum and Eggplant Newsletter ( 12): 76, 1993

Inhibition of vector transmission of Tomato spotted wilt virus (TSWV) by transgenic tomato plants that express the TSWV glycoprotein, G(N). Phytopathology 103(9): 8-8, 2013

Artificial infection of ornamentals with tomato spotted wilt virus (TSWV) and the occurrence of TSWV in Rhineland-Palatinate. Acta Horticulturae ( 377): 141-148, 1994

Expression of a modified nucleocapsid-protein of Tomato spotted wilt virus (TSWV) confers resistance against TSWV and Groundnut ringspot virus (GRSV) by blocking systemic spread. Molecular Plant Pathology 5(4): 309-316, 2004

Continuous high temperature can break the hypersensitivity of Capsicum chinense 'PI 152225' to tomato spotted wilt tospovirus (TSWV). Phytopathologia Mediterranea 35(2): 117-120, 1996

Effects of tomato spotted wilt tospovirus (TSWV) on tobacco thrips are influenced by interactions among TSWV isolate, host plant and temperature. Phytopathology 94(6): S100-S100, 2004

Host list of plants susceptible to tomato spotted wilt virus (TSWV). Research Extension Series, Hawaii Institute of Tropical Agriculture and Human Resources (August): 10, 1987

A soluble form of the Tomato spotted wilt virus (TSWV) glycoprotein G(N) (G(N)-S) inhibits transmission of TSWV by Frankliniella occidentalis. Phytopathology 98(1): 45-50, 2008

Interaction of Tomato Spotted Wilt Tospovirus (TSWV) Glycoproteins with a Thrips Midgut Protein, a Potential Cellular Receptor for TSWV. Phytopathology 88(2): 98, 1998

The susceptibility of tomato and pepper plants to infection with tomato spotted wilt virus - TSWV. Materiay Sesji Instytutu Ochrony Roslin 35(2): 235-237, 1995

The movement protein NSm of tomato spotted wilt tospovirus (TSWV): RNA binding, interaction with the TSWV N protein, and identification of interacting plant proteins. Proceedings of the National Academy of Sciences of the United States of America 97(5): 2373-2378, 2000

Effects of temperature on the transmission of tomato spotted wilt virus TSWV by Frankliniella occidentalis Pergande larvae Effetti della temperatura sulla trasmissione di tomato spotted wilt virus TSWV da parte di neanidi di Frankliniella occidentalis Pergande. Atti del Congresso Nazionale Italiano di Entomologia 17: 509-513, 1994

Inhibition of tomato spotted wilt virus tswv systemic infection in tomato plants by caryophyllales extracts. Fitopatologia Brasileira 14(3-4): 283-287, 1989