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Chapter 23,874

The Fertilizability of Frog Eggs from different Levels of the Female Reproductive Tract

Glick, R.N.; Shaver, J.R.

Experimental Cell Research 32: 615-618

1963


ISSN/ISBN: 0014-4827
PMID: 14099834
DOI: 10.1016/0014-4827(63)90204-0
Accession: 023873118

Quantitative studies have been made on the time after ovulation and of the locale in the genital tract of Rana pipiens when fertilizability is acquired by eggs. Females were injected on each of 2 successive days with pituitary glands, and 48, 54, 60 and 72 hours after injection, were sacrificed and the oviducts removed. Eggs taken from each of 3 segments of equal length of the oviduct were fertilized with normal spermatozoa, and the cleavages were compared to those of eggs taken from the ovisac of the same animal. The data were analyzed statistically in 2 ways 1) Lindquist type 1 analysis, permitting comparisons of fertilizability at different oviducal levels and at different times after initial stimulation to ovulation, and, 2) the sequential Q method was used to compare the order of variation in fertilizability of eggs from the different levels, as well as the variation at the same level at different times. The results indicated: 1) that eggs taken from the upper oviducal level at any time after initial pituitary stimulation, cleave in smaller numbers than those from any more posterior level; 2) eggs taken from any oviducal level or from the ovisac 48 hours after stimulation had a lower fertilizability than eggs from the same regions taken at any later period; 3) between 48 and 54 hours after stimulation, eggs from oviducal levels below the first third become increasingly fertilizable; no significant increase occurs between 54 and 72 hours; 4) eggs from the ovisac inseminated at any of the 4 post-stimulation times cleaved at from 2 to 3 times the rate of eggs from any oviducal level; 5) eggs from the middle and lower oviducal levels were always 2 to 4 cleavage stages ahead of those taken from the ovisac.

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