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Xylanase production by Aspergillus niger LPB 326 in solid-state fermentation using statistical experimental designs



Xylanase production by Aspergillus niger LPB 326 in solid-state fermentation using statistical experimental designs



Food Technology and Biotechnology 46(2): 183-189



Xylanase was produced by Aspergillus niger LPB 326 cultivated on lignocellulosic substrate composed by sugarcane bagasse and soybean meal in solid-state fermentation. The effects of various variables were observed and optimized by applying statistical experimental designs. The best xylanase activity was obtained in a medium containing 10 g of sugarcane bagasse and soybean meal in the ratio of 65 and 35 %, respectively, moistened to 85 % of initial water content with a nutrient salt solution composed of (in g/L): CuSO4 0.4, KH2PO4 1.5 and CoSO4 0.0012, and incubated for 4 days at 30 degrees C. Under these optimized conditions, a xylanase activity of 3099 IU/g of dry matter was obtained.

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Accession: 024147817

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