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A revised medium for rapid growth and bioassays with tobacco tissue cultures



A revised medium for rapid growth and bioassays with tobacco tissue cultures



Physiol Plantarum 15(3): 473-497



A several-fold increase in yield of excised pith or callus cultures of Nicotiana tabacum, variety Wisconsin 38 was obtained by addition of leaf extract to the standard modified White's nutrient medium. In part the increase was due to inorganic constituents of the extract, especially N and K, which could be substituted for by either the ash of the extract or raised levels of N and K salts in the medium. In part the increase was due to organic constituents of the extract which increased both the growth rate and final fresh weight of the cultures. Similar increases in growth rate but not in final yield were obtained by adding gibberellic acid and Braun's supplements of purines and amino acids to a revised medium. A revised medium has been developed with each element provided in sufficient quantity to insure that no increase in yield will result from the introduction of additional amounts in the range ordinarily to be expected in plant tissue extracts, etc. The organic constituents have been retained unchanged except that sucrose has been raised to 3%, myo-inositol has been added as a regular constituent and Edamin has been introduced as an optional constituent. The revised medium is designed for use in bioassays of organic growth factors. It provides for rapid growth rate, increased response to organic growth factors and minimal interference from inorganic and common organic nutrients. In the presence of plant extracts fresh weight yields of up to 35 g on 50 ml of medium have been obtained. Under these conditions water appears to be the limiting factor for growth of the tobacco tissues employed. Aspects of general application, limitations and behavior of plant tissue cultures are discussed.

Accession: 024189129

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