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Alkaline phosphatase in cells cultivated in vitro

Alkaline phosphatase in cells cultivated in vitro

Compt Rend Soc Biol 143(9/10): 731-734

The authors achieved more than 600 cultures of skeletal muscles and subcutaneous connective tissue of chicken embryo (9-12 days old). Cultures were grown on various media (notably plasma 25%, glycosol 25%, embryo extract 25%, serum 25%). Titration was by Gomori's method (using a short fixation and a long incubation in glycerophosphate). A high concn. of phosphatase in nucleoli was noted, higher than in chromosomes. The amt. of phosphatase is related directly to the intensity of growth. Results agreed with those of Brachet- Jeener and Hevesy. Furthermore, cells cultured in vitro retain the ability to synthetize alkaline phosphatase.

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Accession: 024197051

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