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Bioassay for evaluation of oral typhoid vaccine

Bioassay for evaluation of oral typhoid vaccine

Rev Inst Med Trop Sao Paulo 4(5): 302-304

The assay consists essentially of the vaccination by oral route of a group of mice, another like group serving as control. All animals are challenged by the oral route with a standardized living suspension of typhoid bacillus after a certain time, and bled from the heart in due time for bacterial counts. The difference between mean log counts in both groups gives a protective index, since bacteriemia is inversely related to resistance.

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