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Bioautography of antibiotics on thin layer chromatograms


Bioautography of antibiotics on thin layer chromatograms



Journal of Chromatography 26(2): 531-532



ISSN/ISBN: 0021-9673

PMID: 6032164

DOI: 10.1016/s0021-9673(01)98921-6

Chromatographic bioassay of antibiotics against gram positive organisms is described. Bacteria are grown under a glass chromatographic plate with a known amount of oxidant as a growth limiting factor. Oxygen is best used when Streptococcus lactis is used as test organism. When studying antibiotics which are non-inhibitory for this bacterium, Staphylococcus aureus 209 P can be substituted. When S. aureus is used, potassium nitrate should replace O2 as an oxidant in terminal respiration.

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Accession: 024236479

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