Blood glycolysis in experimental hemolytic anemias produced by dyes

Ruumina, V.I.; Sezebrennikova, I.A.; Kleytman, Y.I.

Tr Vses O Va Fiziol Biokhim I Farmakol 3: 95-99


Accession: 024255706

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Hemolytic anemia was produced in experiments in 25 rabbits by subcutaneous injection of 1-2% aqueous solution of picric acid (130 mg/kg), methylene blue (200 mg/kg) - and for the purpose of comparison - by injection of 10% phenylhydrazine (200 mg/kg). Determinations were made of Hb values, erythrocyte (E) and reticulocyte (R) counts, changes in E, sugar and lactic acid (L) contents and the glycolytic properties of E in defibrinated blood. In experiments with methylene blue, the majority of rabbits showed on the second day, increased Hb and E counts: 60-70% of E contained hemoglobinic degenerative bodies. Sugar content rose 1 1/2 times. Formation of L decreased; the rate of its increase after 3 hr. of glycolysis prior to injection of the poison was on the average 52%, after 24 hr. - 33%. After injection of picric acid the values of Hb and E decreased; degenerative bodies appeared in the E. The sugar content increased, and the rise of L on the second day, after 3 hr. of glycolysis decreased, by 18%. The values of Hb and E after injection of phenylhydrazine decreased on the second day, and the glycolytic function of E decreased. In all 3 series of experiments the appearance of a large number of E with degenerated structures coincided with lowering of glycolysis. During the second phase of the anemia, as the damaged forms of E disappeared and the bone marrow function improved (increased reticulocyte count), glycolysis intensity increased and blood sugar content steadily decreased.