Home
  >  
Section 25
  >  
Chapter 24,292

Cell-free protein synthesis directed by coliphage MS2 RNA: synthesis of intact viral coat protein and other products

Nathans, D.

Journal of Molecular Biology 13(2): 521-531

1965

ISSN/ISBN: 0022-2836
PMID: 5325728
DOI: 10.1016/s0022-2836(65)80114-0
Accession: 024291741
Coliphage MS2 RNA directs the synthesis in Escherichia coli extracts of a protein product the tryptic peptides of which correspond to the peptides of the phage coat protein. Fingerprint analysis of the synthetic protein labeled with various mino acids indicated that in each case only those coat protein peptides which contain a particular amino acid were labeled.

PDF emailed within 0-6 h: $19.90




Related References

Eggen, K.; Nathans, D. 1969: Regulation of protein synthesis directed by coliphage MS2 RNA. II. In vitro repression by phage coat protein Journal of Molecular Biology 39(2): 293-305
Igarashi, S.J.; Paranchych, W. 1970: In vitro protein synthesis directed by R17 viral ribonucleic acid. 3. a new assay method for cell-free protein synthesis based on a phenylthiohydantoin procedure Canadian Journal of Biochemistry 48(4): 438-443
Igarashi, S.; Paranchych, W. 1967: In vitro protein synthesis directed by R17 viral ribonucleic acid. I. Initiation of polypeptide synthesis in a purified cell-free system Biochemistry 6(8): 2571-2578
Nathans, D.; Oeschger, M.P.; Polmar, S.K.; Eggen, K. 1969: Regulation of protein synthesis directed by coliphage MS2 RNA. I. Phage protein and RNA synthesis in cells infected with suppressible mutants Journal of Molecular Biology 39(2): 279-292
Mathews, M.; Korner, A. 1970: Mammalian cell-free protein synthesis directed by viral ribonucleic acid European Journal of Biochemistry 17(2): 328-338
Ohtaka, Y.; Spiegelman, S. 1963: Translational Control of Protein Synthesis in a Cell-Free System Directed by a Polycistronic Viral RNA Science 142(3591): 493-497
Igarashi, S.J. 1969: In vitro protein synthesis directed by R17 viral ribonucleic acid. II. on the fate of mRNA in the cell-free system Canadian Journal of Biochemistry 47(12): 1179-1186
Gelfand, D.H.; Hayashi, M. 1969: DNA-dependent RNA-directed protein synthesis in vitro, II. Synthesis of a phi-X-174 coat protein component Proceedings of the National Academy of Sciences of the United States of America 63(1): 135-137
Yamazaki, H.; Kaesberg, P. 1966: Analysis of products of protein synthesis directed by R17 viral RNA Proceedings of the National Academy of Sciences of the United States of America 56(2): 624-631
Verlinden, Y.; Cuconati, A.; Wimmer, E.; Rombau, B. 2002: The viral protein 3CD induces an equilibrium between the viral protein and RNA synthesis in a cell-free system for poliovirus replication Archives of Virology 147(4): 731-744
Eggen, K.; Oeschger, M.P.; Nathans, D. 1967: Cell-free protein synthesis directed by colphage MS2 RNA: sequential synthesis of specific phage proteins Biochemical and Biophysical Research Communications 28(4): 587-597
Terry, P.M.; Ganguly, R.; Ball, E.M.; Banerjee, M.R. 1975: Murine mammary gland RNA directed synthesis of casein in a heterologous cell-free protein synthesis system Cell Differentiation 4(2): 113-122
Ito, K.; Date, T.; Wickner, W. 1980: Synthesis, assembly into the cytoplasmic membrane, and proteolytic processing of the precursor of coliphage M13 coat protein Journal of Biological Chemistry 255(5): 2123-2130
Sugiyama, T.; Stone, H.O. 1969: Protein synthesis directed by an amber coat-protein mutant of the RNA phage MS2 Journal of Molecular Biology 42(1): 97-115
Kozak, M.; Nathans, D. 1972: Differential inhibition of coliphage MS2 protein synthesis by ribosome-directed antibiotics Journal of Molecular Biology 70(1): 41-55
Halperen, S.; Eggers, H.J.; Tamm, I. 1963: Synthesis of ECHO 12 virus coat protein in the absence of coordinated synthesis of viral RNA Jour Cell Biol 19(2): 30a
Danis, C.; Lemay, G. 1993: Protein synthesis in different cell lines infected with orthoreovirus serotype 3: inhibition of host-cell protein synthesis correlates with accelerated viral multiplication and cell killing Biochemistry and Cell Biology 71(1-2): 81-85
Gessesse, B.; Nagaike, T.; Nagata, K.; Shimizu, Y.; Ueda, T. 2018: G-Protein Coupled Receptor Protein Synthesis on a Lipid Bilayer Using a Reconstituted Cell-Free Protein Synthesis System Life 8(4)
Suzuki, T.; Ito, M.; Ezure, T.; Shikata, M.; Ando, E.; Utsumi, T.; Tsunasawa, S.; Nishimura, O. 2007: Protein prenylation in an insect cell-free protein synthesis system and identification of products by mass spectrometry Proteomics 7(12): 1942-1950
Kim, J.-O.; Kim, J.-O.; Kim, W.-S.; Oh, M.-J. 2015: Development of a Recombinant Protein Vaccine Based on Cell-Free Protein Synthesis for Sevenband Grouper Epinephelus septemfasciatus Against Viral Nervous Necrosis Journal of Microbiology and Biotechnology 25(10): 1761-1767