Centrifugation of filiform particles in a density gradient: The separation of the yellows virus of beets from leaf homogenates

Mundry, K.Wolfgang; Schneider, F.

ZEITSCHR NATURFORSCH 11b(9/10): 573-578

1956


Accession: 024294090

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Abstract
Density gradient centrigugation in sucrose solutions has proved useful in experiments to separate the sugar beet yellows virus from normal constituents of leaf homo-genates. After dialysis at pH 6.8 in a combined veronal-phosphate buffer following centrifugation the virus had lost practically none of its serological activity and only a little of its infectivity. Density gradient centrifugation as a means of preventing compact sediments is especially useful in attempts to isolate thread-like particles.