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Control of haptoglobin metabolism, III. Purification of the hemoglobin-haptoglobin subunit and the hemo-globin-haptoglobin complex from human serum of the haptoglobin 1-1 type



Control of haptoglobin metabolism, III. Purification of the hemoglobin-haptoglobin subunit and the hemo-globin-haptoglobin complex from human serum of the haptoglobin 1-1 type



Proc Jap Acad 43(4): 332-337



The Hb-Hp [hemoglobin-haptoglobin] "subunit" and the Hb-Hp complex were purified from human blood of the Hp 1 -1 type by ammonium sulfate fraction, gel filtration on a Sephadex G-100 column, and chromatography on a DEAE-Sephadex G-100 column. Each purified sample was homogeneous in the ultracentrifuge. Disc electrophoresis revealed a minor contaminant less than 3% of the total protein. Disc electrophoresis results suggested that a reversible change from the Hb-Hp complex to the Hb-Hp "subunit" does not occur at least in vitro. It is concluded that the Hb-Hp "subunit II" is identical with the Hb-Hp complex.

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Accession: 024404954

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