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Cresyl violet staining in exfoliative gynecologic cytology


Cresyl violet staining in exfoliative gynecologic cytology



Obstetrics and Gynecology 15: 702-710



ISSN/ISBN: 0029-7844

PMID: 14422016

A short staining technique for routine light microscopy is described which uses a saturated solution of cresyl violet (C. I. 877) in acetate buffer 0.01 M at pH 4.0 with subsequent simultaneous dehydration and differentiation by acetone alone. Five different commercially available brands of cresyl violet were analyzed by partition chromatography. The method was tested on smears from 300 patients. These comprised cervical scrapings and vaginal smears of all classifications (I - V). Smears exhibiting irradiation, menopause and pregnancy effects were also included. Paraffin and frozen tissue sections from mouse organs and from different transplantable tumors were used. The advantage of this single-dye method is the simplicity of the procedure with a short staining time (in cytological smears 3 minutes; in frozen and paraffin sections 40-60 seconds) and uniformly consistent results. The varying intensity of staining approximates the concentration of nucleic acids and proteins. The cytoplasm and nucleus stand out in good contrast. Details of cytoplasmic and nuclear structures are brought out through the excellent transparency of the staining. Cresyl violet excels as a sharp nuclear stain with remarkable demonstration of the degree of hyper-chromasia. The nucleoli appear in a metachromatic shade. Lightly stained red blood cells do not obscure other elements. Clear representation of cellular structures together with information about the distribution of metachromatic and basophilic substances make this method valuable for gynecologic cytology.

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Accession: 024417846

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