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Differential Staining Of Bone Tissue Elements By Silver Nitrate Staining Of Blocks Of Decalcified Bone



Differential Staining Of Bone Tissue Elements By Silver Nitrate Staining Of Blocks Of Decalcified Bone



Anatomical Record 151: 125-131



Bone can be differentially stained en-bloc by fixing in 10% formalin, decalcifying in dilute nitric-acid and placing in an ammoniacal silver-nitrate solution. The block is then infiltrated and embedded in gelatin for serial sectioning while frozen. Structures range from colorless to yellow, through different shades of brown or reddish-brown, to black. This range is seen among different osteons, and even within a single osteon. Color changes within the same structure can be observed easily in serial sections. Collagen fibers, Sharpey's fibers and assorted cell nuclei are also stained, but cement lines are not. Canaliculi usually do not stain, but occasionally stain vividly. Very darkly stained areas show little histological detail. Some of the characteristics of bone stained by this technique suggest that a relationship exists between the extent of mineralization and degree of staining. Similar features of x-ray photomicrographs of undecalcified bone sections support such a relationship.

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Accession: 024491273

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PMID: 14278707

DOI: 10.1002/ar.1091510204


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