Section 25
Chapter 24,553

Effect of monochromatic ultraviolet light on macromolecular synthesis in Escherichia coli

Hanawalt, P.; Setlow, R.

Biochimica et Biophysica Acta 41: 283-294


ISSN/ISBN: 0006-3002
PMID: 14399416
DOI: 10.1016/0006-3002(60)90011-1
Accession: 024552613

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Tracer techniques were used to follow the synthesis of protein and nucleic acids in Escherichia coli strains B and 15T-, after irradiation of exponentially growing cultures with monochromatic ultraviolet light (u.v.). Deoxyribonucleic acid (DNA) synthesis is most sensitive to u.v.; the effect is a delay in the initiation of P32 incorporation into DNA. Doses above 150 ergs/mm2 at 2652 A result in cessation of DNA synthesis for a period greater than one normal division time. The incorporation of S35 into protein and of P32 into RNA is linear with time after u.v. doses which stop DNA synthesis. Protein synthesis is inhibited more than RNA synthesis, and the dose-effect curves indicate a higher multiplicity for the effect of u.v. on RNA synthesis than on protein synthesis. Action spectra for the effect of u.v. on protein synthesis and RNA synthesis are similar and follow a nucleic acid absorption spectrum.

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