Effect of the volume of semen and number of spermatozoa on the fertilization rate in artificial insemination of pigs
Zhivotnovodstvo 9: 53-66
Forty females, intended to be fattened after delivery of a single litter, were fertilized 24 to 26 hours after the beginning of the female immobility reflex with semen diluted in 100 ml of glucose-citrate-egg yolk diluent containing 5, 10 and 20 billion spermatozoa. In a 2d experiment, the effect of the volume of semen on the fertilizability in the presence of the same number of spermatozoa was studied. Twenty-four to 48 hours later, the oviducts and ovaries were removed surgically from all the pigs and the number of opened follicles, as well as the ratio of zygotes to unfertilized egg cells in the oviducts, was determined. The highest fertilization rate (64%) was observed at a dosage of 10 billion spermatozoa per 100 ml dilute semen, 77 opened follicles, 24 egg cells and 43 zygotes being detected in 6 pigs. Increasing the number of spermatozoa to 20 billion did not increase the percent fertilization, but decreasing it to 5 billion decreased the rate of fertilization to 24%. One hundred ml should be considered to be the optimum volume of semen. Fractionated administration of semen has no advantages over non-fractionated administration.