Effect of thyroid hormones, somatotrophin, insulin and corticosteroids on synthesis of collagen in granulation tissue both in vivo and in vitro

Mikkonen, L.; Lampiaho, K.; Kulonen, E.

Acta Endocrinologica 51(1): 23-31


ISSN/ISBN: 0001-5598
PMID: 5951905
DOI: 10.1530/acta.0.0510023
Accession: 024566297

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The effect of hormonal factors on the collagen synthesis in experimental granulation tissue of rats was studied in vivo by assessing the development of the tensile strength and in vitro by measuring the capacity of collagen synthesis from C 14-proline by incubated granuloma slices. The hormonal agents were either administered in vivo or added to the incubation media. When hormones were given in vivo, only cortisol affected the tensile strength of the granuloma markedly, but when the capacity of collagen synthesis was assessed, it was found that somatotrophin slightly increased and thyroxine decreased the synthesis. The effect of glucocorticoids, if any, was to increase the conversion of labelled proline into hydroxy-proline. In those experiments in which the hormonal factors were added to the incubation media, thyroxine and particularly insulin, increased the incorporation of C14-proline into collagen. Somatotrophin decreased the incorporation of proline in these experiments, presumably by artifact reactions.