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Electron Microscopy Of Post-Mortem Changes In The Rat Myocardium



Electron Microscopy Of Post-Mortem Changes In The Rat Myocardium



Anatomical Record 147: 261-272



An electron microscopic study was made on specimens of rat myocardium taken at various intervals after death, in order to determine the extent and character of autolytic change occurring during the first ten hours. Specimens were taken immediately after death and at 15 minutes, one hour, three hours, five hours and ten hours after death, fixed in 1% phosphate-buffered osmium tetroxide and examined with the electron microscope. The first noticeable change was a rapid depletion of glycogen. The specimens taken one hour or more after death show a longer sarcomere length indicating a more relaxed state. This is believed to reflect the inability of these fibers to contract upon stimulation by the initial contact with the fixative. Nuclei, when encountered, showed more chromatin clumping than in fresh tissue, but the nuclear membranes were intact. Very little change was noted in the mitochondria, sarcoplasmic reticulum, or other cellular components.

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Accession: 024602011

Download citation: RISBibTeXText

PMID: 14065938

DOI: 10.1002/ar.1091470209


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