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Enzyme studies with mutant mammalian cells






Journal of Biological Chemistry 235: 1765-1768

Enzyme studies with mutant mammalian cells



Accession: 024620059

PMID: 14416836



Related references

Eder, J.P.; Chan, V.T.; Niemierko, E.; Teicher, B.A.; Schnipper, L.E., 1993: Conditional expression of wild-type topoisomerase II complements a mutant enzyme in mammalian cells. Alterations in the amino acid composition, phosphorylation pattern, or intracellular levels of topoisomerase II have been associated with resistance to antineoplastic agents whose effects are mediated through interactions with this enzyme. To deve...

Johansson, L.; Arscott, L.David.; Ballou, D.P.; Williams, C.H.; Arnér, E.S.J., 2006: Studies of an active site mutant of the selenoprotein thioredoxin reductase: the Ser-Cys-Cys-Ser motif of the insect orthologue is not sufficient to replace the Cys-Sec dyad in the mammalian enzyme. We have mutated the redox active C-terminal motif, Gly-Cys-Sec-Gly, of the mammalian selenoprotein thioredoxin reductase (TrxR) to mimic the C-terminal Ser-Cys-Cys-Ser motif of the non-selenoprotein orthologue of Drosophila melanogaster (DmTrxR)....

Suzuki F., 1973: Genetic studies of somatic mammalian cells in vitro part 1 isolation of mutant cells by a replica plating method. Journal Of Radiation Research: 103

Hanada, K.; Nishijima, M.; Fujita, T.; Kobayashi, S., 2000: Specificity of inhibitors of serine palmitoyltransferase (SPT), a key enzyme in sphingolipid biosynthesis, in intact cells: A novel evaluation system using an SPT-defective mammalian cell mutant. In the present study, we demonstrate a model cell system for evaluating the specificity of inhibitors of serine palmitoyltransferase (SPT), the enzyme that catalyzes the first step of sphingolipid biosynthesis. The LY-B strain is a Chinese hamster...

Hanada, K.; Nishijima, M.; Fujita, T.; Kobayashi, S., 2000: Specificity of inhibitors of serine palmitoyltransferase (SPT), a key enzyme in sphingolipid biosynthesis, in intact cells. A novel evaluation system using an SPT-defective mammalian cell mutant. In the present study, we demonstrate a model cell system for evaluating the specificity of inhibitors of serine palmitoyltransferase (SPT), the enzyme that catalyzes the first step of sphingolipid biosynthesis. The LY-B strain is a Chinese hamster...

Chen, M.; Beck, W.T., 1993: Teniposide-resistant CEM cells, which express mutant DNA topoisomerase II alpha, when treated with non-complex-stabilizing inhibitors of the enzyme, display no cross-resistance and reveal aberrant functions of the mutant enzyme. We have examined the effects of a group of DNA topoisomerase II (topo II) inhibitors, merbarone, aclarubicin, SN22995, RP60475F, and fostriecin, in CCRF-CEM cells and two sublines, CEM/VM-1 and CEM/VM-1-5, that were selected for increasing resista...

Chu, E.H.; Giles, N.G., 1958: Comparative chromosomal studies on mammalian cells in culture. I. The HeLa strain and its mutant clonal derivatives. Journal of the National Cancer Institute 20(2): 383-401

Anonymous, 1958: Comparative chromosomal studies on mammalian cells in culture L The HeLa strain and its mutant clonal derivatives

Sweetman, L.; Nyhan, W.L., 1972: Further studies of the enzyme composition of mutant cells in X-linked uric aciduria. Archives of Internal Medicine 130(2): 214-220

Darroudi, F.; Natarajan, A.T., 1992: Studies on the origin of chromosomal aberrations induced by ionizing radiation Utilization of repair deficient mammalian mutant cells. International Journal of Radiation Biology 62(3): 375