Evolution of the chromosome complement of the swine cell line IB-RS-2

D.C.stro, M.P.; Koseki, I.

Arq Inst Biol sao Paulo 34(4): 285-294


Accession: 024645211

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A continuation of the history of the swine kidney cell line, IB-RS-2, as well as the evolution of its chromosome complement, from the 56th to the 154th cell transfers are given. Up to the 75th transfer, there was a prevalence of cells with 37 centromeres belonging to 1.1 type. After the recovery of the cultures from a dramatic cell necrosis, the prevalent karyotypes had 38 centromeres. These karyotypes could be found in the cell clones derived from the parental cell population. It would appear that the 7 clones whic could be analyzed are descendants of cells with 38 chromosomes, type 2.2: 2 of them, C-12 and C-19 are type 2.2, but C-19 presents a secondary constriction in one of the largest GIV-XX chromosomes not found in C-12; 5 of them have a new large subacrocentric chromosome (SA). C-16, C-17, C-18 and C-60 (karyotype 2.2.1) have also a microchromo-some which is not found in C-13 (karyotype 2.2.2). These 5 clones had been originated from a cell of 2.2 karyotype, through a recombination between one of the largest GIV-XX and one of the GVI chromosomes, giving rise to the large SA and to the microchromosome. Afterwards, in C-13, this microchromosome was lost.