+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Fluorescent cell counting as an assay method for respiratory syncytial virus



Fluorescent cell counting as an assay method for respiratory syncytial virus



Journal of Virology 1(3): 494-499



The fluorescent cell-counting technique was applied to the enumeration of cell-infecting units of respiratory syncytial (RS) virus in human fetal diploid (HFD) cover-slip cell cultures; it was a sensitive, precise, and rapid assay method. Approximately 2 hr was required for maximal adsorption of RS virus to HFD cell monolayers. However, about 15% of the infectious virus in the inoculum remained unadsorbed; this percentage was not significantly reduced even when the adsorption period was extended to 5 hr. A linear relationship between virus concentration and the number of fluorescent cells existed over a range of 1.2 log(10) units. Variation of the mean of replicate determinations in a single experiment was approximately 7.5%. The distribution of single infected HFD cells on cover-slip cell cultures corresponded with the calculated frequencies of the Poisson distribution. The Chi square test for the extent of fit was calculated for several experiments, and the value of P was never less than 0.5. The addition of immune serum after virus adsorption effectively inhibited the development of detectable levels of viral antigen in secondarily infected cells.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 024703627

Download citation: RISBibTeXText

PMID: 4918238


Related references

An improved respiratory syncytial virus neutralization assay based on the detection of green fluorescent protein expression and automated plaque counting. Virology Journal 9: 253, 2012

Detection of human metapneumovirus and respiratory syncytial virus by duplex real-time RT-PCR assay in comparison with direct fluorescent assay. Clinical Microbiology and Infection 16(10): 1568-1573, 2010

Indirect fluorescent assay for respiratory syncytial virus antibodies. Abstracts of the Annual Meeting of the American Society for Microbiology 83: C142, 1983

Development of an improved microneutralization assay for respiratory syncytial virus by automated plaque counting using imaging analysis. Virology Journal 2: 84, 2005

A comparison of Binax NOW to viral culture and direct fluorescent assay testing for respiratory syncytial virus. Diagnostic Microbiology and Infectious Disease 49(4): 265-268, 2004

Development of a High-Throughput Respiratory Syncytial Virus Fluorescent Focus-Based Microneutralization Assay. Clinical and Vaccine Immunology 24(12):, 2017

Development of real-time fluorescent reverse transcription loop-mediated isothermal amplification assay with quenching primer for influenza virus and respiratory syncytial virus. Journal of Virological Methods 267: 53-58, 2019

Production of respiratory syncytial virus antigens during growth in human hep 2 cultures part 1 fluorescent antibody assay. Experimental Medicine and Microbiology (English Translation of Medycyna Doswiadczalna i Mikrobiologia) 24(2): 120-127, 1972

A comparison of Thermo Electron RSV OIA to viral culture and direct fluorescent assay testing for respiratory syncytial virus. Journal of Clinical Virology 32(3): 224-228, 2005

An Assay Method For Respiratory Syncytial Virus. British Journal of Experimental Pathology 44: 473-480, 1963

Fluorescent cell-counting assay of yellow fever virus. Journal of Infectious Diseases 116(1): 33-40, 1966

Assay of variola virus by the fluorescent cell-counting technique. Applied Microbiology 13(6): 865-871, 1965

Quantitative Assay Of Psittacosis Virus By The Fluorescent Cell-Counting Technique. Virology 23: 203-208, 1964

Assay of wound tumor virus by the fluorescent cell counting technique. Virology 37(4): 667-677, 1969

Detection of respiratory syncytial virus in naso pharyngeal secretions by enzyme linked immuno sorbent assay comparison with fluorescent antibody technique. Journal of Medical Virology 10(4): 273-282, 1982