+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Formation of the nicotinic acid analogue of diphosphopyridine nucleotide after nicotinamide administration



Formation of the nicotinic acid analogue of diphosphopyridine nucleotide after nicotinamide administration



Journal of Biological Chemistry 234(8): 2161-2168



A pyridine nucleotide isolated from the livers of mice given injections of nicotinamide has been identified as the nicotinic acid analogue of diphosphopyridine nucleotide (DPN). Evidence that this nicotinic acid analogue of DPN is an intermediate in the synthesis of DPN from both nicotinamide and nicotinic acid, as well as from other pyridine compounds, rests on a study of (a) the time course of formation of both DPN and the nicotinic acid analogue of DPN after injection of nicotinamide, (b) the rate of labeling of the nicotinic acid analogue of DPN after injection of radioactive nicotinamide, and (c), the formation of the nicotinic acid analogue of DPN after administration of graded amounts and different types of pyridine compounds. Azaserine inhibited the synthesis of DPN from nicotinamide, and allowed some accumulation of the nicotinic acid analogue in the absence of any appreciable synthesis of DPN. Azaserine also caused a marked decline in liver DPN levels in normal mice. Substrate inhibition was produced with nicotinic acid in the synthesis of DPN and its nicotinic acid analogue. Large doses of nicotinic acid also inhibited the synthesis of the nicotinic acid analogue from nicotinamide and [beta]-picoline. Pyridine-3-aldehyde and pyridine-3-carbinol were rapidly oxidized to nicotinic acid by the mouse.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 024711934

Download citation: RISBibTeXText

PMID: 13673032


Related references

The nicotinic acid analogue of diphosphopyridine nucleotide. Journal of Biological Chemistry 231(2): 685-694, 1958

Synthesis of desamido-diphosphopyridine-nucleotide by an exchange enzymatic reaction between diphosphopyridine-nucleotide and nicotinic acid. Gazzetta Chim Ital 88(3): 320-330, 1958

Synthesis of diphosphopyridine nucleotide from nicotinic acid. S P Colowick and N O Kaplan, Editors Methods in Enzymology Vol VI p 345-352, 1963

Utilization of tryptophan, nicotinamide and nicotinic acid as precursors for nicotinamide nucleotide synthesis in isolated rat liver cells. British Journal of Nutrition 59(2): 279-287, 1988

Accumulation of oxidized diphosphopyridine nucleotide in mouse liver after administration of reduced diphosphopyridine nucleotide. Archives of Biochemistry and Biophysics 136(1): 106-111, 1970

Influence of nicotic acid and nicotinamide on diphosphopyridine nucleotide (DPN) Synthesis in ascites tumor cells. Biochemical Pharmacology 8: 335-336, 1961

Biosynthesis of diphosphopyridine nucleotide. III. Nicotinic acid mononucleotide pyrophos-phorylase. Journal of Biological Chemistry 236: 525-530, 1961

Biosynthesis of nicotinamide adenine dinucleotide in the frog liver Effect of nicotinamide and nicotinic acid administration. Revue Roumaine de Biologie Serie de Biologie Animale, 251: 45-50, 1980

The isolation and properties of the isonicotinic acid hydrazide analogue of diphosphopyridine nucleotide. Journal of Biological Chemistry 209(2): 467-484, 1954

Formation of [nicotinamide-²H₃]NAD⁺ from [²H₄]nicotinamide and [²H₄]nicotinic acid in human HepG2N cells and involvement of ²H/¹H exchange at the redox site of NAD⁺/NADH. Journal of Nutritional Science and Vitaminology 60(1): 17-21, 2014

The reduction of the acetylpyridine analogue of diphosphopyridine nucleotide by dihydrodiphoaphopyridine nucleotide. Jour Biol Chem 235(8): 2498-2501, 1960

Preparation of nicotinic acid mono nucleotide from nicotinamide mono nucleotide by enzymatic deamidation. Mccormick, Donald B And Lemuel D Wright (Edited By) Methods in Enzymology Vol Xviii Vitamins And Coenzymes, Part B Xxi+857p Illus Academic Press: New York, N Y , U S A ; London, England 192-197, 1971

Inhibition of diphosphopyridine nucleotide-requiring enzymes by nicotinamide. Journal of Biological Chemistry 189(1): 361-369, 1951

Nicotinamide inhibition of tri- and diphosphopyridine nucleotide-linked dehydrogenases. Jour Biol Chem 199(2): 493-504, 1952