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Formation of tuberculin by washed tubercle bacilli in citrate solution. IX. Comparative study on some biological properties of streptomycin-resistant and streptomycin-sensitive cells of various strains of Mycobacterium tuberculosis with special reference to tuberculin production



Formation of tuberculin by washed tubercle bacilli in citrate solution. IX. Comparative study on some biological properties of streptomycin-resistant and streptomycin-sensitive cells of various strains of Mycobacterium tuberculosis with special reference to tuberculin production



Japanese Jour Tuberc 5(1/2): 35-43



Streptomycin-resistant mutants were produced in vitro from a variety of strains of human and bovine types of M. tuberculosis. They were tested for their tuberculin-producing property in comparison with their parent drug-sensitive strains. Among the streptomycin-resistant mutants, the one derived from human tubercle bacillus, Aoyama B strain, possessed a greatly reduced ability to produce tuberculin-active substance(s) in a citrate solution, and in addition, that "citrate-tuberculin" solution thus obtained from this variant contained no detectable free amino acids in contrast with the "citrate-tuberculin" solutions obtained from other strains studied.

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