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Further studies on the amino acid incorporating system of rat liver microsomes



Further studies on the amino acid incorporating system of rat liver microsomes



Journal of Biological Chemistry 233(3): 643-649



The ability of pyrophosphate to disaggregate the microsomal ribonucleic acid (RNA) -rich particles has been utilized to obtain several nucleoprotein fractions plus a residual microsome fraction (RM) containing about 15% of the original RNA and consisting essentially of the membranous components of microsomes. These fractions were distinct with respect to their chemical composition (protein and RNA content) and the metabolic activities of their protein and RNA moieties. The rates of incorporation of C14-leucine into the protein of these fractions were compared in vivo and in vitro. The results obtained in vitro clearly resemble the labeling pattern observed during the first minute of amino acid incorporation in vivo. The redistribution of RNA and protein labeled in vitro among the microsome fractions was studied. In the presence of an ATP-generating system considerable counts and RNA orginially present in the most active fraction (M2) subsequently appeared mainly in Fraction Ml and in the soluble fraction. This redistribution represented an outward movement (or passage into solution) of the labeled nucleoprotein of Fraction M2. Preliminary experiments on the incorporation in vivo of P32- inorganic phosphate into the RNA moieties of the microsomal fractions are also reported and the significance of the results discussed.

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Accession: 024731496

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PMID: 13575429


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