+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector



Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector



Journal of Biotechnology 139(3): 211-213



A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 024741413

Download citation: RISBibTeXText

PMID: 19124048

DOI: 10.1016/j.jbiotec.2008.12.007


Related references

Genetic recombination in Bacillus subtilis: effect of dhelD on DNA repair and homologous recombination. Journal of Bacteriology 183(19): 72-7, 2001

Genetic recombination in Bacillus subtilis 168: effect of DeltahelD on DNA repair and homologous recombination. Journal of Bacteriology 183(19): 5772-5777, 2001

Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination. Journal of Bacteriology 180(13): 3405-3409, 1998

Homologous recombination using bacterial artificial chromosomes. Cold Spring Harbor Protocols 2015(2): 180-190, 2015

Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination. Proceedings of the National Academy of Sciences of the United States of America 90(17): 8118-8122, 1993

A method to invert DNA segments of the Bacillus subtilis 168 genome by recombination between two homologous sequences. Bioscience Biotechnology and Biochemistry 60(5): 773-778, 1996

A new method for generating modifications in bacterial artificial chromosomes by homologous recombination in E coli. American Journal of Human Genetics 67(4 Suppl. 2): 190, 2000

Retrofitting bacterial artificial chromosomes by homologous recombination into enhanced BAC vectors for functional studies. Biotechniques 35(5): 1054-6 1058, 2003

Modification of bacterial artificial chromosomes through chi-stimulated homologous recombination and its application in zebrafish transgenesis. Proceedings of the National Academy of Sciences of the United States of America 95(9): 5121-5126, 1998

Retrofitting bacterial artificial chromosomes by homologous recombination into enhanced Bac vectors for functional studies. BioTechniques 35(5): 1054-1058, 2003

A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coli. Nucleic Acids Research 29(3): E14, 2001

Integrative bacterial artificial chromosomes for DNA integration into the Bacillus subtilis chromosome. Journal of Microbiological Methods 125: 1-7, 2016

Molecular cloning of heterologous chromosomal dna by recombination between a plasmid vector and a homologous resident plasmid in bacillus subtilis. Molecular and General Genetics 177(3): 459-468, 1980

Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus. Frontiers in Microbiology 7: 2110, 2016

Genetic Recombination of Transforming Deoxyribonucleic Acid Molecules with the Recipient Genome and Among Themselves in Protoplasts of Bacillus subtilis. Journal of Bacteriology 92(2): 455-463, 1966