Germination of oat pollen on artificial media

Wallace, A.T.; Karbassi, P.

Crop Sci 8(4): 506-507


Accession: 024748717

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Freshly collected oat pollen was germinated in Petri dishes on media containing 13 g sucrose, 1.7 g raffinose, 20 mg boric acid, 25 mg calcium nitrate, 3 g bacto-agar, and 85 ml double-distilled water. Germination is improved if the dishes containing the media are "aged" 2 to 10 days in a refrigerator. The pollen germinates within 1 to 2 min. after it comes in contact with the media. Within another 5 to 6 min. the tubes usually have grown to their maximum length, 125 to 150 microns, at which time the ends burst and exude their contents. Modifying agents proved to be ineffective in preventing the bursting.