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Hemolysis and blood group determination. Experimental studies of the occurrence of hemolysis


Hemolysis and blood group determination. Experimental studies of the occurrence of hemolysis



ARCH KLIN CHIR 152(Kongressber ): 477-508



The author describes his so-called biological test and urges its use in transfusions. It consists in injection of 5-10 cc. of donor blood into the recipient. If no immediate symptoms follow preliminary injection, the transfusion is carried out. The occurrence of characteristic symptoms immediately after the test injection indicates incompatibility and another donor must be selected. According to the author, hemolytic reactions occur almost immediately after injection of a small quantity of incompatible blood. In such cases the presence of hemoglobin in the serum can always be demonstrated. Hemoglobin is present in the urine only after a much larger quantity of incompatible blood is injected. During the period before compatibility tests were made with Landsfeiner's isoagglutinin reaction, the author was guided in selection of donors by the biological test and during this time he observed 50 instances of hemolytic reactions with but 1 fatality. Experimental evidence in man and animals is adduced to show that hemolysis occurs in vivo instantaneously while in vitro it is present but occasionally, and is a slow process.

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