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Histochemical studies of normal salivary glands. I. Histochemical demonstration of succinic dehydrogenase. II. Demonstration of esterase. III. Demonstration of alkaline phosphatase



Histochemical studies of normal salivary glands. I. Histochemical demonstration of succinic dehydrogenase. II. Demonstration of esterase. III. Demonstration of alkaline phosphatase



Jour Osaka Univ Dental Soc 4(2): 421-455



Succinic dehydrogenase activity in tne salivary glands of the mouse, rat, guinea-pig, dog and human being was examined by the neotetrazolium reaction. Several coarse or fine dark purple granules were found in the protoplasm of the secretory cells of all the submaxillary glands. Coarse granules were present in all the parotid glands, but all the sublingual glands contained fewer granules. The strongest activity was in the intralobular duct cells, which were stained black or dark purple. This seems to be correlated with the fact that numerous secretion granules were also found in this area. The interlobular ducts showed less activity, being stained dark purple or purplish. Esterase activity in the salivary glands of the mouse, rat, guinea-pig, rabbit and dog was studied by the Nachlas-Seligman method. The glandular cells of the submaxillary glands generally contained fine purple granules. The protoplasm was generally stained reddish purple. In the dog, the glandular cells were stained diffusely and were light in colour, though the base of the cells was dark. In the rabbit, the cells were light red and contained no granules, and therefore no esterase. In all the animals very strong esterase activity was observed in the interlobular ducts, but the intralobular ducts showed less activity. The rat showed the highest esterase activity in the glandular cells of the sublingual glands; they were diffusely stained, but contained no granules. The base of the cells was especially darkly stained. Rather high activity was also observed in the glandular cells of the sublingual glands of the rabbit and dog, which contained fine dark purple granules. In the mouse and guinea-pig, the glandular cells contained fine light reddish purple granules. The ducts in the sublingual glands were generally darkly stained. The glandular cells of the parotid glands were dark reddish purple, though the esterase activity was, in general, lower than in the other two glands. In the rabbit no esterase activity was found in the parotid or submaxillary glands; the glandular cells were light red and contained no granules. The ducts of the parotid glands generally showed more esterase activity than the secretory cells. Alkaline phosphatase activity in the salivary glands of the mouse, rat, guinea-pig, rabbit and dog was studied by the azo dye method ([alpha]-naphthyl-phosphate and diazo blue B). In the submaxillary glands high activity was observed generally at the base of the glandular cells. The protoplasm was not stained. No protoplasm was not stained. No phosphatase activity was found in the submaxillary glands of guinea-pigs or rabbits. In the sublingual glands, the base of the secretory cells and the protoplasm showed diffuse and moderate phosphatase activity in all species except the mouse. In the rat there was some activity at the base of the cells, but none in the protoplasm. The highest activity was found in the dog. In the parotid glands, no activity was found in the mouse or the dog, slight activity in the rat, and moderate and diffusely distributed activity in the guinea-pig and rabbit. In all 3 salivary glands, moderate, or even high phosphatase activity was seen in the capillaries, except in the submaxillary glands of the guinea-pig and in the sublingual glands of the rat, which showed no phosphatase activity.

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