Histological and histochemical investigations of the adrenal cortex of the white mouse during the process of its regeneration

Belka-Grzybek, A.

Folia Biologica 13(2): 157-171

1965


ISSN/ISBN: 0015-5497
PMID: 5843295
Accession: 024791993

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Abstract
The left adrenal gland of sexually mature male white mice of strain A was removed under ether anaesthesia, the right one being subjected to thermal shock using a thermocauter. The adrenal glands of the control animals as well as all the left adrenal glands removed during the operational procedure were used as controls. The operated animals were killed after 24 hr., 2, 5, 10, 15, 30, 60 and 90 days. The sectioned adrenal glands were examined morphologically (with hematoxylin-eosin, and Feulgen's method) and histochemically to determine the content of ascorbic acid (Bacchus's method), steroids (Khanolkar's method in Penar's modification), and lipids (Lilli's method). In the injured adrenal gland, numerous morphogenetic changes had taken place in the surviving cortical and connective tissue indicating an attempt at organ regeneration. During the 1st period after the shock, a violent decrease in the content of ascorbic acid was observed. After 5 days the reaction is back to normal in the parts untouched by the shock. In the regenerating zone nearest the injury, the weak reaction lasts for 2 months before the specificity of the reaction is restored in all parts of the adrenal gland. A violent decrease in the content of lipids occurs during the 1st period after the shock. Here too, after 2 months, the reaction is back to normal in all zones of the adrenal gland. The intensity of the reaction for steroid hormones greatly decreased during the 1st 2 weeks of regenerative processes, showing almost no change until about 2 months after the shock.