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Im-munological study of a neuro-inductive protein in amphibians

Im-munological study of a neuro-inductive protein in amphibians

Kumamoto J Sci Ser B Sect 2 Biol 8(1): 13-19

Ribonucleoprotein [RNP] was separated from guinea pig liver (GpL-RNP) and 9-day chick embryos (CE-RNP) by the streptomycin method. Rabbit antiserum obtained against GpL-RNP was used for studies of the immunological nature and inductive activity of the ribonucleoprotein. The inductive activity of antigen-antibody complexes was also studied. In agar immunodiffusion tests, 2 or 3 components were recognized in the GpL-RNP. Diffusion of anti-GpL-RNP serum against CE-RNP produced 1 thick precipitin band common to GpL-RNP, but the main, heavy precipitin line obtained in the homologous reaction was not observed. The antigen, crude antibody globulin, and antigen-antibody complexes were examined for their inductive effects on the presumptive epidermis of Triturus pyrrhogaster gastrulae. The antigen showed a strong neuro-inductive effect; the inductive activity of the crude antibody globulin was negligible. The precipitates obtained by the immune reaction between antigen and antiserum did not show any inductive activity. The inductive capacity of the antigen is greatly depressed by the immune reaction with the crude antibody globulin. The inhibition of inductive capacity was discussed from a viewpoint that active sites of inductor proteins might be partially similar to antigen active sites.

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