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Incorporation of amino acids into polyribosome-associated protein in cytoplasmic extracts of poliovirus-infected HeLa cells


Incorporation of amino acids into polyribosome-associated protein in cytoplasmic extracts of poliovirus-infected HeLa cells



Biochemical and Biophysical Research Communications 15(3): 290-295



ISSN/ISBN: 0006-291X

PMID: 4158131

DOI: 10.1016/0006-291x(64)90162-7

The characteristic large polyribosomes, which play a functional role in viral protein synthesis within poliovirus-infected HeLa cells also appear to be the principal, if not sole site of the amino acid incorporation catalyzed in vitro by cytoplasmic extracts of such cells. In analogy with the results in the intact cell, a portion of the polyribosome-associated protein labeled in vitro reacts with antibody directed against subunits of the virus capsid. At least the bulk of the capacity of extracts of infected cells to incorporate amino acids can be ascribed to polyribosomes which are associated with cytoplasmic structures sedimentable in 20 minutes at 40, 000 g. Such structures also contain most of the RNA polymerase responsible for the synthesis of viral RNA, and are the site of viral RNA synthesis both in vitro and in the intact cell (D. Baltimore and J. E. Darnell, personal communication).

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Accession: 024838658

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Related references

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