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Induction of apogamy in megagameto-phytes of Zamia integrifolia

Induction of apogamy in megagameto-phytes of Zamia integrifolia

Amer J Bot 52(10): 993-999

Ovules of Zamia integrifolia Ait. were dissected and mega-gametophytes and embryos excised and cultured. Induction of apoga-mous, haploid roots and leaves by auxin and kinetin, in combination with glutamine, aspargine and alanine, was observed. The organs resembled their normal diploid counterparts, 2, 4-D at 1 ppm was more effective than 1 ppm IAA, but less effective than 5 ppm IAA. The highest percentage of apogamy was 59% after 5 months of culture. In several cultures embryoids were formed both by diploid excised embryos and by haploid megagametophytes.

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Accession: 024844597

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DOI: 10.2307/2440128

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