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Influence of sampling time and test-plant reading time on results of indexing prune trees for ringspot virus



Influence of sampling time and test-plant reading time on results of indexing prune trees for ringspot virus



Adv Frontiers Plant Sci 7: 53-67



Three series of Italian prune trees (P. domestica) in commercial orchards were variously and repeatedly indexed on peach seedlings in a greenhouse and field nursery, and on Shirofugen flowering cherry trees (P. serrulata) in the field from 1957-1961. Although results varied among the 3 test methods, and within each method, best results were obtained in the greenhouse in March, in the nursery in May, and with Shirofugen trees in July. In terms of total numbers of positive sources detected per sample, the greenhouse test was superior to the nursery test and to the Shirofugen test, but Shirofugen reacted more uniformly to individual inoculum sources in individual tests. Variation of efficiency of ringspot virus detection, in both indicator species, appeared to result primarily from incomplete distribution of ringspot virus in donor prune trees in all times; but also from inconsistencies in test plants due to the physiologic variations of differing conditions and stages of growth, and from differences in virulence characteristics of the virus inocula.

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