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Investigations of the physiology of wine yeasts. II. Conditions of growth of further auxo-auto-trophic Swiss culture yeasts



Investigations of the physiology of wine yeasts. II. Conditions of growth of further auxo-auto-trophic Swiss culture yeasts



Antonie Van Leeuwenhoek Jour Microbiol And Serol 18(1): 31-44



Results are reported concerning 2 more Swiss wine yeasts for fermentation at low temps., the sporogenous races Salenegg (red wine) and Herrliberg (white wine). The growth was examined in nutrient solns. containing glucose, mineral salts and ammonium sulfate or casein hydrolysale as a source of N. The Beckmann spectrophotometer was used to measure growth. Of the growth factors tested (+)-biotin, pyridoxine (adermin), thiamin and pantothenic acid, added singly and in various combinations, had no stimulating action, whereas addition of meso-inositol in amts. greater than 0.25-2.5 mg./1000 ml. produced a marked increase in growth. Max. growth was attained with 25 mg. of meso-inositol per 1000 ml. The stimulating effect of meso-inositol was not increased by (+)-biotin, added singly or in combination with pantothenic acid. Both yeasts were successfully subcultured about 150 times in the chemically defined medium without exogenous supply of vitamins. These results confirm the authors' view that it is incorrect to generalize the theory of auxo-heterotrophy of tame yeasts.

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