Klinische und experimentelle Untersuchungen uber Hamolyse durch Gallen-saure und ihre Hemmung durch Serum
Zcitschr Ges Exp Med 57(1/2): 234-252
Intravenous injections of bile acids in 16 persons (including 7 with jaundice) produced a highly variable amount of hemolysis. To learn something of the individual differences in the sensitivity of the blood toward bile acids, the conditions of bile acid hemolysis were studied in test tubes. Within wide limits, there was a proportional relationship between the quantities of red blood cells and amount of bile acids necessary to produce hemolysis. The percentage concentration of the bile acids, within wide limits, played no role. The resistance of washed erythrocytes to bile acids was found fairly constant as to the beginning and end of hemolysis. The hemolysis is inhibited by the presence of serum, this inhibition showing definite quantitative differences in various diseases and also during the course of the same disease. It is a non-specific phenomenon and runs parallel with the sedimentation rate. If this occurs quickly, the degree of binding [amt. of bile acids which can be so bound by 100 cc. serum as to produce no hemolysis of 10 cc. washed erythrocytes] is small. It apparently depends on the degree of dispersion of the serum albumin present[long dash]the albumin:globulin ratio in the blood serum.