Section 26
Chapter 25,036

Morphological and cytochemical changes of cytoplasmic granules during development of Hydrodictyon reticulatum

Yamagishi, H.

Japanese Society of Plant Physiologists, Editors Studies on Microalgae and Photosynthetic Bacteria 35-40


Accession: 025035009

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The morphological and cytochemical changes of cytoplasmic granules in H. reticulatum in the course of development (Stages 1 -5) were studied. Many spherical granules are found in the cytoplasm. These granules are divided into 3 groups; granules which are stained with neutral red; granules showing fluorescence on treatment with Nile blue; and electrondense granules. These granules share the same locality in the cells, and are similar in size (0.5-1.0 [mu]) and shape, but their identity or interrelationship remains undecided. These cytoplasmic granules increase in number in the course of development, reach a maximum at the middle phase of Stage 3 and then decrease. The fluorescence intensity of the cytoplasmic ground substance stained with berberine sulfate is strong at Stage 3 and weak at the end of Stage 4. The berberine-induced fluorescence increment of the supernatant fraction (10,000 x g, 20 min. increases at early stages of development, arrives at a maximum at a late phase of Stage 3 and then decreases. The cause of these changes is discussed.