Morphological and cytochemical changes of cytoplasmic granules during development of Hydrodictyon reticulatum
Japanese Society of Plant Physiologists, Editors Studies on Microalgae and Photosynthetic Bacteria 35-40
The morphological and cytochemical changes of cytoplasmic granules in H. reticulatum in the course of development (Stages 1 -5) were studied. Many spherical granules are found in the cytoplasm. These granules are divided into 3 groups; granules which are stained with neutral red; granules showing fluorescence on treatment with Nile blue; and electrondense granules. These granules share the same locality in the cells, and are similar in size (0.5-1.0 [mu]) and shape, but their identity or interrelationship remains undecided. These cytoplasmic granules increase in number in the course of development, reach a maximum at the middle phase of Stage 3 and then decrease. The fluorescence intensity of the cytoplasmic ground substance stained with berberine sulfate is strong at Stage 3 and weak at the end of Stage 4. The berberine-induced fluorescence increment of the supernatant fraction (10,000 x g, 20 min. increases at early stages of development, arrives at a maximum at a late phase of Stage 3 and then decreases. The cause of these changes is discussed.