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Morphological peculiarities of the actinomycetes. Actinomyces lavendulae and Actinomyces aureofaciens under prolonged cultivation in a continuous culture

Morphological peculiarities of the actinomycetes. Actinomyces lavendulae and Actinomyces aureofaciens under prolonged cultivation in a continuous culture

Mikrobiologiya Ftransl] 30(3): 351-357

Observations were carried out on the morphology of 2 actinomyces species, viz., Actinomyces lavendulae and Actinymyces aureofaciens, grown in a streming medium. It proved possible to keep A. lavendulae and A. aureofaciens in the phase of intense growth throughout the cultivation period (840 and 172 hours, respectively). The bulk of the mycelium exhibited features appropriate to young hyphae. Rejuvenescence of the abnormal aging culture of A. aureofaciens preliminarily grown under stationary conditions could be achieved by a continuous supply of fresh nutrient medium and elimination of the culture fluid. In Actinomyces cultivated in a streaming medium hyphae of a different physiological age are always revealed in the culture. The effect of certain physico-chemical factors was demonstrated, such as pH, quality of the corn extract, rate of supply of the culture fluid, etc., on the appearance of hyphae of different age in a streaming medium.

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