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Photoreactivation of induced mutation and inactivation of Escherichia coli exposed to various wave lengths of monochromatic ultraviolet radiation



Photoreactivation of induced mutation and inactivation of Escherichia coli exposed to various wave lengths of monochromatic ultraviolet radiation



Journal of Bacteriology 75(2): 190-198



Mutations to streptomycin independence in E. coli strain SD-4 and to purine independence in E. coli strain 82/r were measured in investigations of the action spectra and photoreactivation (PR) of inactivation and mutation after monochromatic UV irradiation. The inactivation and mutation action spectra for both strains were of the nucleic acid type, with broad maxima around 2600 A. A linear mutation dose response is obtained with E. coli strain SD-4 at all wave lengths tested whereas E. coli strain 82/r displays exponential mutation dose curves. An effect of cell concentration per mutant assay plate on the number of mutations phenotypically expressed was observed for 82/r but not for SD/4. Photoreactivation (PR) of inactivation and mutation occurs in both 82/r and SD-4 after exposure to wave lengths from 2378 to 2967 A. The fraction of the UV effects not photoreactivable in SD-4 is the same for all wave lengths tested, and the PR kinetics observed for inactivation and mutation are similar. The proportion of the UV effect that is photoreactivable is significantly greater for mutation than for inactivation at all wave lengths studied in SD-4.

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Accession: 025235281

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PMID: 13513581


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